CIISB project proposal

Equipment requested

VP-iTC^?
Auto PEAQ-ITC^?

Experiment details

(VP-ITC only)

Time of measurement required: days

If you plan to use other liquids than water-based buffers, please specify in "Other information" field.

(Auto PEAQ-ITC only)

Number of samples:	Number of replicates per sample:
Blank measurement required: Yes No	Total measurements expected:

Do you request measurement at different temperatures?:

If Yes, please specify, experiments are routinely performed at 25°C

Type of experiments (Auto PEAQ-ITC only)

Standard titration experiments
Competitive titration experiments for low or ultrahight affinity interactions
Single titration injection (continuous titration)

Method of evaluation (Auto PEAQ-ITC only)

Automated evaluation using in-build software fitting (one independent binding site model and/or one independent binding site model with fixed stoichiometry)
Expert evaluation (one or two independent binding site model, cooperativity, competitive binding, kinetics)

Other information, or if more description is needed than is covered by the form:

Equipment requested:

VP-DSC
Auto PEAQ-DSC

Time of measurement required (for VP-DSC only): days

Number of samples (including multiplicates and buffer scans, for Auto PEAQ-DSC only):

Type of experiments planned (temperature range, scan rate, number of repeated scans/down scans, etc.): Other information, or if more description is needed than is covered by the form:

Used instrument for measurement:

Monolith NT.115
Monolith NT.115 Pico

Number of runs required^?:

Used label (for Monolith NT.115): Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Number of runs required:

Extinction coef. at 280 nm (for Prometheus NT.48): Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Time of measurement required: days

Sensor chips for measurement:

I will use my own chip
I need a chip provided by CF

If you want sensor chips to be supplied by CF, please specify the chip type and the number of chips you request:

Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Time of measurement required: days

Biosensors for measurement:

I will use my own biosensors
I need biosensors provided by CF

Amine Reactive Second-Generation (AR2G) Biosensors
Protein A (ProA) Biosensors
Streptavidin (SA) Biosensors
Anti-HIS (HIS2) Biosensors

If you want sensors to be supplied by CF, please specify the sensor type and the number of sensors you request:

Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Time of measurement required: days

Sensor chips for measurement:

I will use my own chip
I need a chip provided by CF

Note: the Core Facility provides the unmodified sensor chips with gold layer only. If you require the chip coating services, please specify in "Other information" field.
Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Number of samples:

Number of total measurements expected:

Type of experiments:

Sedimentation velocity (1 day/experiment)
Sedimentation equilibrium (4 days/experiment)

Note that max 7 samples may be measured in one experiment. (for some experiment types no more than 3 may be measured in one experiment)

Other information, or if more description is needed than is covered by the form:

Used instrument for measurement:

DelsaMax (cuvette measurements)
Spectrolight 600 (plate reader measurements)

Time of measurement required: hours

Temperature of experiments: Type of experiments: Other information, or if more description is needed than is covered by the form:

Time of measurement required: hours

Cuvette types required:

Need to change the typical configuration

Please specify in the “Other information” field below. This requires technical assistence.
(Standard configuration includes: peltier, standard cuvette, CD detector, fluorescence monochromator)

Type of experiments: Other information, or if more description is needed than is covered by the form:

Number of samples:

Requested technique

Standard screen set-up Specify screens required in "Other information" field.	Number of screen plates per sample required:
Plate storage and inspection Standard time of plate inspection is one month (4 weeks), plates are typically stored for additional 5 months without inspection.	Prolonged inspection period: 4 +: weeks
Optimization of crystallization	Number of screen plates per sample required:
Advanced crystallization techniques Specify in "Other information" field.

Other information, or if more description is needed than is covered by the form:

SAXS service required:

Measurement of non-biological nanostructures
Basic characterization of biological macromolecules by SAXS
Determination of a 3-D shape of biological macromolecules by SAXS

Mode of service:

Full service (measurement, data processing and data analysis provided by CF, only for biological molecules)
Measurement without analysis of SAXS data

Numerical quantification of requirement for biological SAXS characterization:

Estimated number of samples for biological SAXS characterization for a selected period of time:

Time period:

week month year

Diffraction service for single crystal sample(s) required:

Preliminary non robotized X-ray tests (e.g test of cryoprotectant, test protein/salt, test of diffraction quality of single crystal) with biological sample
Robotized test of diffraction quality of protein crystals
Collection of diffraction data with crystals of biological macromolecules
Collection of diffraction data with non-biological single crystals without data analysis
Data collection and solving of the crystal structures with non-biological single crystals

Mode of service:

Full service (measurement, data processing and data analysis provided by CF)
Measurement without analysis of diffraction data

Numerical quantification of requirement for non-biological single crystal diffraction:

Estimated number of samples for non-biological X-ray diffraction experiments for a selected period of time:

Time period:

week month year

First name:		Surname:
Email:		Phone number:
Position:	Researcher Ph.D. student MSc student

First name:		Surname:
Email:		Phone number:
Position:	Researcher Ph.D. student MSc student

same as applicant
First name:		Surname:
Email:		Phone number:
Invoicing address:
University Public research organization Industry company
Organization:
Address^?:		Country:

Sample name:
Biosafety level:^?	1 2
Number of aliquotes/grids:
Concentration of sample stock^?:
Total molecular mass or size^?:
Buffer or solvent composition:
Delivery conditions:	RT 4C -20C -80C LN2
Storage conditions:	RT 4C -20C -80C LN2
Additional sample information^?:

Aggregation state (solid/liquid):
Molecular weight:
solvent/buffer/pH:
Temperature (typical/max allowed):
Concentration:
Stability:
Specify if the sample is isotopically labelled:	natural isotopic abundance 15N 13C 2H other
Comments:

Applicant information:

Principle investigator:

Invoicing address:

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):^?

Background in the lab and preliminary data (from 500 to 3000 characters):^?

Files and figures (PDFs and images accepted):^?

Project objectives and experimental plan (from 250 to 3000 characters):^?

Select core facilities and proceed to their specific subforms:

CEITEC - Biomolecular Interactions and Crystallography

Sample(s) information:

General service information:

Choose required services and fill in particular details for each.

Equipment requested

Experiment details

Type of experiments (Auto PEAQ-ITC only)

Method of evaluation (Auto PEAQ-ITC only)

Requested technique

Numerical quantification of requirement for biological SAXS characterization:

Numerical quantification of requirement for non-biological single crystal diffraction:

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Required service(s): (select requested service, multiple choices possible.)

CEITEC - Nanobiotechnology

CEITEC - Proteomics

Sample(s) information:

Sample origin^?:

Sample history^?:

Gel electrophoresis:

Required service(s):

CEITEC - Josef Dadok National NMR Centre

Sample(s) information:

BIOCEV - Biophysical techniques

Choose required services and fill in required details.

BIOCEV - Crystallization of proteins and nucleic acids

BIOCEV - Diffraction techniques

Choose required services and fill in required details.

BIOCEV - Structural mass spectrometry

BIOCEV - Protein production

BIOCEV sample(s) information

Research project title:
Acronym:	(max. 10 characters) Will be used as project identifier.

Time needed to prepare and deliver samples:
Estimated measuring time required (days):

Number of crystals (estimate):
Expected phasing method (options: MR, MIR, SAD, other):

Applicant information:

Principle investigator:

Invoicing address:

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):?

Background in the lab and preliminary data (from 500 to 3000 characters):?

Files and figures (PDFs and images accepted):?

Project objectives and experimental plan (from 250 to 3000 characters):?

Select core facilities and proceed to their specific subforms:

CEITEC - Biomolecular Interactions and Crystallography

Sample(s) information:

General service information:

Choose required services and fill in particular details for each.

Equipment requested

Experiment details

Type of experiments (Auto PEAQ-ITC only)

Method of evaluation (Auto PEAQ-ITC only)

Requested technique

Numerical quantification of requirement for biological SAXS characterization:

Numerical quantification of requirement for non-biological single crystal diffraction:

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Required service(s): (select requested service, multiple choices possible.)

CEITEC - Nanobiotechnology

CEITEC - Proteomics

Sample(s) information:

Sample origin?:

Sample history?:

Gel electrophoresis:

Required service(s):

CEITEC - Josef Dadok National NMR Centre

Sample(s) information:

BIOCEV - Biophysical techniques

Choose required services and fill in required details.

BIOCEV - Crystallization of proteins and nucleic acids

BIOCEV - Diffraction techniques

Choose required services and fill in required details.

BIOCEV - Structural mass spectrometry

BIOCEV - Protein production

BIOCEV sample(s) information

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):^?

Background in the lab and preliminary data (from 500 to 3000 characters):^?

Files and figures (PDFs and images accepted):^?

Project objectives and experimental plan (from 250 to 3000 characters):^?

Sample origin^?:

Sample history^?: