CIISB project proposal

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):^?

Background in the lab and preliminary data (from 500 to 3000 characters):^?

Files and figures (PDFs and images accepted):^?

Project objectives and experimental plan (from 250 to 3000 characters):^?

CEITEC - Biomolecular Interactions and Crystallography

Type of access:

Single project access
BAG (block allocation group) access - internal users only

Sample(s) information:

Sample name:

Sample description^?:

Do the samples present any risk to human health and/or environment?:

Yes No

Class of risk:

1 2 3

Is the sample recombinant?:

Yes No

If Yes, please specify details in the Other specification field

Source of origin:

The sample is:

active virus virulence factor toxin prion protein

Other specifications:

General service information:

Are you interested in data evaluation service (if relevant)?:

Yes No

If yes, please specify in which method

Are you interested in training in data processing (if relevat?):

Yes No

If yes, please specify in which method

Are you interested in expert consulting assistance?:

Yes No

If yes, please specify in which method

Choose required services and fill in particular details for each.

Calorimetric titration

Equipment requested

VP-iTC^?
Auto PEAQ-ITC^?

Experiment details

(VP-ITC only)

Time of measurement required: days

If you plan to use other liquids than water-based buffers, please specify in "Other information" field.

(Auto PEAQ-ITC only)

Number of samples:	Number of replicates per sample:
Blank measurement required: Yes No	Total measurements expected:

Do you request measurement at different temperatures?:

If Yes, please specify, experiments are routinely performed at 25°C

Type of experiments (Auto PEAQ-ITC only)

Standard titration experiments
Competitive titration experiments for low or ultrahight affinity interactions
Single titration injection (continuous titration)

Method of evaluation (Auto PEAQ-ITC only)

Automated evaluation using in-build software fitting (one independent binding site model and/or one independent binding site model with fixed stoichiometry)
Expert evaluation (one or two independent binding site model, cooperativity, competitive binding, kinetics)

Other information, or if more description is needed than is covered by the form:

Differential Scanning Calorimetry

This type of experiment is supposed to be managed by the user itself after special training.

Equipment requested:

VP-DSC
Auto PEAQ-DSC

Time of measurement required (for VP-DSC only): days

Number of samples (including multiplicates and buffer scans, for Auto PEAQ-DSC only):

Type of experiments planned (temperature range, scan rate, number of repeated scans/down scans, etc.): Other information, or if more description is needed than is covered by the form:

Microscale Thermophoresis

This type of experiment is supposed to be managed by the user itself after special training.

Used instrument for measurement:

Monolith NT.115
Monolith NT.115 Pico

Number of runs required^?:

Used label (for Monolith NT.115): Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Differential scanning fluorimetry (Prometheus NT.48)

This type of experiment is supposed to be managed by the user itself after special training.

Number of runs required:

Extinction coef. at 280 nm (for Prometheus NT.48): Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Surface plasmon resonance (Biacore S200)

This type of experiment is supposed to be managed by the user itself after special training.

Time of measurement required: days

Sensor chips for measurement:

I will use my own chip
I need a chip provided by CF

If you want sensor chips to be supplied by CF, please specify the chip type and the number of chips you request:

Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Biolayer interferometry (Octet RED96e)

This type of experiment is supposed to be managed by the user itself after special training.

Time of measurement required: days

Biosensors for measurement:

I will use my own biosensors
I need biosensors provided by CF

Amine Reactive Second-Generation (AR2G) Biosensors
Protein A (ProA) Biosensors
Streptavidin (SA) Biosensors
Anti-HIS (HIS2) Biosensors

If you want sensors to be supplied by CF, please specify the sensor type and the number of sensors you request:

Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Surface plasmon resonance (SFR Imaging multichannel system)

This type of experiment is supposed to be managed by the user itself after special training.

Time of measurement required: days

Sensor chips for measurement:

I will use my own chip
I need a chip provided by CF

Note: the Core Facility provides the unmodified sensor chips with gold layer only. If you require the chip coating services, please specify in "Other information" field.
Type of experiments planned: Other information, or if more description is needed than is covered by the form:

Analytical ultracentrifugation

Number of samples:

Number of total measurements expected:

Type of experiments:

Sedimentation velocity (1 day/experiment)
Sedimentation equilibrium (4 days/experiment)

Note that max 7 samples may be measured in one experiment. (for some experiment types no more than 3 may be measured in one experiment)

Other information, or if more description is needed than is covered by the form:

Dynamic light scattering

This type of experiment is supposed to be managed by the user itself after special training.

Used instrument for measurement:

DelsaMax (cuvette measurements)
Spectrolight 600 (plate reader measurements)

Time of measurement required: hours

Temperature of experiments: Type of experiments: Other information, or if more description is needed than is covered by the form:

CD / fluorescence measurement

This type of experiment is supposed to be managed by the user itself after special training.

Time of measurement required: hours

Cuvette types required:

1 mm path
2mm path
5mm path
10mm path
5mm fluorescence compatible
10mm fluorescence compatible
Other - please specify in Other infomation below

Need to change the typical configuration

Please specify in the “Other information” field below. This requires technical assistence.
(Standard configuration includes: peltier, standard cuvette, CD detector, fluorescence monochromator)

Type of experiments: Other information, or if more description is needed than is covered by the form:

Protein Crystallization

Number of samples:

Requested technique

Standard screen set-up Specify screens required in "Other information" field.	Number of screen plates per sample required:
Plate storage and inspection Standard time of plate inspection is one month (4 weeks), plates are typically stored for additional 5 months without inspection.	Prolonged inspection period: 4 +: weeks
Optimization of crystallization	Number of screen plates per sample required:
Advanced crystallization techniques Specify in "Other information" field.

Other information, or if more description is needed than is covered by the form:

Small-angle X-ray scattering

SAXS service required:

Measurement of non-biological nanostructures
Basic characterization of biological macromolecules by SAXS
Determination of a 3-D shape of biological macromolecules by SAXS

Mode of service:

Full service (measurement, data processing and data analysis provided by CF, only for biological molecules)
Measurement without analysis of SAXS data

Numerical quantification of requirement for biological SAXS characterization:

Estimated number of samples for biological SAXS characterization for a selected period of time:

Time period:

week month year

X-ray diffraction

Diffraction service for single crystal sample(s) required:

Preliminary non robotized X-ray tests (e.g test of cryoprotectant, test protein/salt, test of diffraction quality of single crystal) with biological sample
Robotized test of diffraction quality of protein crystals
Collection of diffraction data with crystals of biological macromolecules
Collection of diffraction data with non-biological single crystals without data analysis
Data collection and solving of the crystal structures with non-biological single crystals

Mode of service:

Full service (measurement, data processing and data analysis provided by CF)
Measurement without analysis of diffraction data

Numerical quantification of requirement for non-biological single crystal diffraction:

Estimated number of samples for non-biological X-ray diffraction experiments for a selected period of time:

Time period:

week month year

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Sample name:
Biosafety level:^?	1 2
Number of aliquotes/grids:
Concentration of sample stock^?:
Total molecular mass or size^?:
Buffer or solvent composition:
Delivery conditions:	RT 4C -20C -80C LN2
Storage conditions:	RT 4C -20C -80C LN2
Additional sample information^?:

Type of access:

Single project access
BAG (block allocation group) access

Required service(s): (select requested service, multiple choices possible.)

access to microscopes^?
TEM imaging
SEM imaging
sample vitrification
resin embedding
FIB-SEM tomography
initial structural screening^?
initial cryo-EM screening^?
cryo-FIB lamella preparation^?
single particle analysis data collection
cryo-electron tomography data collection
electron diffraction tomography
data analysis^?

CEITEC - Nanobiotechnology

Type of analysis:

Cells - mechanical properties
Cells - imaging
Biomolecules - imaging
Nano-objects - imaging
Raman-AFM combined microscopy
Raman microscopy
Electrochemical measurements
Nanodeposition system
Fluorescence scanner
SPR biosensor
Multi-electrode array (MEA)

Sample(s) description^?: Required service(s):

Full service (measurement and analysis) Measurement only Data processing only

CEITEC - Proteomics

It is strongly recommended to discuss the project with head of Proteomics CF prior submission.

Sample(s) information:

Organism:

Sample origin^?:

Sample history^?:

Gel electrophoresis:

1D
2D

pl range: % T: Staining:

Required service(s):

Protein fractionation/separation (1D, 2D, GE, IEF, LC)
Intact mass analysis
Protein identification
Characterisation of protein modifications
Absolute and relative protein quantification

CEITEC - Josef Dadok National NMR Centre

Sample(s) information:

Aggregation state (solid/liquid):
Molecular weight:
solvent/buffer/pH:
Temperature (typical/max allowed):
Concentration:
Stability:
Specify if the sample is isotopically labelled:	natural isotopic abundance 15N 13C 2H other
Comments:

Type of NMR experiments^?: Equipment required^?:

Time needed to prepare and deliver samples:
Estimated measuring time required (days):

Previous NMR measurements on the sample:

none
1D 1H
2D Homonuclear
2D Heteronuclear
Heteronuclear 3D NMR
other

Please provide previously measured spectra if available.

Required services:

Full service
Full measurement and assistance with analysis
Full measurement without analysis
Assistance with measurement and analysis
Assistance with masurement
No assistance

BIOCEV - Biophysical techniques

If at any point of your measurements you need to use a different CMS core facility, you don´t need to fill out another request form. In such a case, please contact the facility you have requested access for and they will instruct you further.

Type of access:

Single project access
BAG (block allocation group) access

Choose required services and fill in required details.

Surface plasmon resonance (SPR)

What kind of sensor chip is needed:

NanoDSF assay

Are Trp or Tyr amino acids present:

Labeled microscale thermophoresis

Which dye is needed for protein labelling:

Label free microscale thermophoresis

Isothermal Titration Calorimetry

Differential Scanning Calorimetry

UV/visible spectrometry

Circular Dichroism

Which buffer is used:

Dynamic light scattering

Fourier-transform infrared (FTIR) spectrometry

Spectrofluorimetry

Absorbance and Fluorescence measurements in plates

Characterization by BLI interaction of biomolecules

Mass Photometry

Other special requests:

BIOCEV - Crystallization of proteins and nucleic acids

Type of access:

Single project access
BAG (block allocation group) access

Please estimate how many hours, days, or individual measurements are planned:
Required services:

Robotic setup of 96-well crystalliziation plates
Manual setup of crystallization plates
Manual setup of crystallization plates under an inert atmosphere
Automated monitoring of crystallization in the crystallization hotel
Crystal handling and preparation for diffraction experiments
Crystal handling and preparation for diffraction experiments in oxygen free conditions
In drop DLS analysis of protein samples and crystallization experiments
Vitrification of grids using the Vitrobot

Other special requests:

BIOCEV - Diffraction techniques

Type of access:

Single project access
BAG (block allocation group) access

Choose required services and fill in required details.

Small angle X-ray scattering

Small angle X-ray scattering (SAXS) measurement

Number of samples (estimate):

SAXS data processing

Assistance with SAXS data interpretation

Measurement of SAXS data at synchrotron radiation sources

Number of samples (estimate):

Single crystal X-ray diffraction

In-situ (in the crystallisation plates) testing of crystal diffraction

Number of plates (estimate):

Measurement of single crystal diffraction data

Testing of difraction quality (estimate number of crystals):

Complete dataset measurement:

Number of crystals (estimate):
Expected phasing method (options: MR, MIR, SAD, other):

Crystal dehumidifying experiment

Number of days (estimate):

Diffraction data processing

Assistance to solve a 3D structure

Measurement of X-ray diffraction data sets at synchrotron radiation sources

Number of crystals (estimate):

X-ray fluorescence measurement

Other special requests:

BIOCEV - Structural mass spectrometry

Type of access:

Single project access
BAG (block allocation group) access

Required services(s):

Precise molecular weight determination
Mass spec data interpretation
MALDI analysis
ESI electrospray analysis
MS sample preparation and handling
Hydrogen deuterium exchange with MS
Chemical cross-linking with MS
Native electrospray
Fast photochemical oxidation of proteins
Single protein identification from gel/solution
Proteins identification/quantification

Special requests:

BIOCEV - Protein production

Required services(s):

Cloning
Protein expression (large-scale)
Protein purification
Small-scale expression and solubility test
Method development

BIOCEV sample(s) information

Sample name:

Sample description^?:

Do the sample present any risk to human health and/or environment?:

Yes No

If Yes, please specify details in the Other specification field Class of risk:

1 2 3

Source of origin: Is the sample recombinant?:

Yes No

If Yes, please specify expression host:

The sample is:

active virus virulence factor toxin prion protein

Other specifications:

First name:		Surname:
Email:		Phone number:
Position:	Researcher Ph.D. student MSc student

First name:		Surname:
Email:		Phone number:
Position:	Researcher Ph.D. student MSc student

same as applicant
First name:		Surname:
Email:		Phone number:
Invoicing address:
University Public research organization Industry company
Organization:
Address^?:		Country:

Applicant information:

Principle investigator:

Invoicing address:

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):^?

Background in the lab and preliminary data (from 500 to 3000 characters):^?

Files and figures (PDFs and images accepted):^?

Project objectives and experimental plan (from 250 to 3000 characters):^?

Select core facilities and proceed to their specific subforms:

CEITEC - Biomolecular Interactions and Crystallography

Sample(s) information:

General service information:

Choose required services and fill in particular details for each.

Equipment requested

Experiment details

Type of experiments (Auto PEAQ-ITC only)

Method of evaluation (Auto PEAQ-ITC only)

Requested technique

Numerical quantification of requirement for biological SAXS characterization:

Numerical quantification of requirement for non-biological single crystal diffraction:

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Required service(s): (select requested service, multiple choices possible.)

CEITEC - Nanobiotechnology

CEITEC - Proteomics

Sample(s) information:

Sample origin^?:

Sample history^?:

Gel electrophoresis:

Required service(s):

CEITEC - Josef Dadok National NMR Centre

Sample(s) information:

BIOCEV - Biophysical techniques

Choose required services and fill in required details.

BIOCEV - Crystallization of proteins and nucleic acids

BIOCEV - Diffraction techniques

Choose required services and fill in required details.

BIOCEV - Structural mass spectrometry

BIOCEV - Protein production

BIOCEV sample(s) information

Research project title:
Acronym:	(max. 10 characters) Will be used as project identifier.

Applicant information:

Principle investigator:

Invoicing address:

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):?

Background in the lab and preliminary data (from 500 to 3000 characters):?

Files and figures (PDFs and images accepted):?

Project objectives and experimental plan (from 250 to 3000 characters):?

Select core facilities and proceed to their specific subforms:

CEITEC - Biomolecular Interactions and Crystallography

Sample(s) information:

General service information:

Choose required services and fill in particular details for each.

Equipment requested

Experiment details

Type of experiments (Auto PEAQ-ITC only)

Method of evaluation (Auto PEAQ-ITC only)

Requested technique

Numerical quantification of requirement for biological SAXS characterization:

Numerical quantification of requirement for non-biological single crystal diffraction:

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Required service(s): (select requested service, multiple choices possible.)

CEITEC - Nanobiotechnology

CEITEC - Proteomics

Sample(s) information:

Sample origin?:

Sample history?:

Gel electrophoresis:

Required service(s):

CEITEC - Josef Dadok National NMR Centre

Sample(s) information:

BIOCEV - Biophysical techniques

Choose required services and fill in required details.

BIOCEV - Crystallization of proteins and nucleic acids

BIOCEV - Diffraction techniques

Choose required services and fill in required details.

BIOCEV - Structural mass spectrometry

BIOCEV - Protein production

BIOCEV sample(s) information

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):^?

Background in the lab and preliminary data (from 500 to 3000 characters):^?

Files and figures (PDFs and images accepted):^?

Project objectives and experimental plan (from 250 to 3000 characters):^?

Sample origin^?:

Sample history^?: