Project proposal
Fields highlighted in red are compulsory. The form can be submitted only after all the required information is provided. Field marked with ? contain additional description, place the cursor over the label to see the help.
 (max. 10 characters) Will be used as project identifier.

Applicant information:

            
            

Principle investigator:

Invoicing address:

            

Scientific background of the project (from 500 to 6000 characters - the extent of the proposal should correspond to the complexity of the requested service):?

Background in the lab and preliminary data (from 500 to 3000 characters):?

Files and figures (PDFs and images accepted):?

Project objectives and experimental plan (from 250 to 3000 characters):?

Select core facilities and proceed to their specific subforms:











CEITEC - Biomolecular Interaction and Crystalliziation



Sample(s) information:

   


        
            
        
If Yes, please specify details in the Other specification field

                

General service information:

    
        
If yes, please specify in which method
    
        
If yes, please specify in which method
    
        
If yes, please specify in which method

Choose required services and fill in particular details for each.

Equipment requested



Experiment details

(VP-ITC only)
   days
If you plan to use other liquids than water-based buffers, please specify in "Other information" field.

(AutoITC200 only)
   
   
    
        
   
If Yes, please specify, experiments are routinely performed at 25°C

Type of experiments (AutoiTC200 only)





Method of evaluation (AutoiTC200 only)




This type of experiment is supposed to be managed by the user itself after special training.
   days
If you plan to use other liquids than water / water-based buffers, please specifiy in "Other information" field.

This type of experiment is supposed to be managed by the user itself after special training.


   
This type of experiment is supposed to be managed by the user itself after special training.
   
This type of experiment is supposed to be managed by the user itself after special training.
   days



If you want sensor chips to be supplied by CF, please specify the chip type and the number of chips you request:
This type of experiment is supposed to be managed by the user itself after special training.
   days


  • Amine Reactive Second-Generation (AR2G) Biosensors
  • Protein A (ProA) Biosensors
  • Streptavidin (SA) Biosensors
  • Anti-HIS (HIS2) Biosensors
If you want sensors to be supplied by CF, please specify the sensor type and the number of sensors you request:
This type of experiment is supposed to be managed by the user itself after special training.
   days


Note: the Core Facility provides the unmodified sensor chips with gold layer only. If you require the chip coating services, please specify in "Other information" field.
   
   


Note that max 3 samples may be measured in one experiment.

This type of experiment is supposed to be managed by the user itself after special training.


   hours
This type of experiment is supposed to be managed by the user itself after special training.
   hours








Please specify in the “Other information” field below. This requires technical assistence.
(Standard configuration includes: peltier, standard cuvette, CD detector, fluorescence monochromator)

   

Requested technique


Specify screens required in "Other information" field.
   

Standard time of plate inspection is one month (4 weeks), plates are typically stored for additional 5 months without inspection.
   weeks

   

Specify in "Other information" field.

CEITEC - X-ray Diffraction and Bio-SAXS Core Facility




For Single project access, please also provide sample information.

Choose required services and fill in particular details for each.

Mode of service:

SAXS characterization of non-biological nanostructures is provided only as a measurement, without any advanced analysis of resulting SAXS data.

Numerical quantification of requirement for non-biological SAXS characterization:

   
    
            





Numerical quantification of requirement for biological SAXS characterization:

   
    
            





Numerical quantification of requirement for non-biological single crystal diffraction:

   
    
            






Numerical quantification of requirements for biological single crystal diffraction:

   
    
            

Biological sample(s) information:

   






If yes, please specify in the Other information field

    
                
    
        


Non-biological sample(s) information:

   

    
        
If yes, please specify in the Other information field

Is the sample expected to be:

    
            

CEITEC - Cryo-electron Microscopy and Tomography

Sample(s) information:

Sample name:
Biosafety level:?
        
Number of aliquotes/grids:
Concentration of sample stock?:
Total molecular mass or size?:
Buffer or solvent composition:
Delivery conditions:
                    
Storage conditions:
                    
Additional sample information?:


Required service(s): (select requested service, multiple choices possible.)














CEITEC - Nanobiotechnology












            

CEITEC - Proteomics

It is strongly recommended to discuss the project with head of Proteomics CF prior submission.

Sample(s) information:

Organism:

Sample origin?:

Sample history?:

Gel electrophoresis:



pl range: % T: Staining:

Required service(s):






CEITEC - Josef Dadok National NMR Centre

Sample(s) information:













Please provide previously measured spectra if available.






BIOCEV - Biophysical techniques

If at any point of your measurements you need to use a different CMS core facility, you don´t need to fill out another request form. In such a case, please contact the facility you have requested access for and they will instruct you further.


Choose required services and fill in required details.

BIOCEV - Crystallization of proteins and nucleic acids

If at any point of your measurements you need to use a different CMS core facility, you don´t need to fill out another request form. In such a case, please contact the facility you have requested access for and they will instruct you further.



Please estimate how many hours, days, or individual measurements are planned:







BIOCEV - Diffraction techniques

If at any point of your measurements you need to use a different CMS core facility, you don´t need to fill out another request form. In such a case, please contact the facility you have requested access for and they will instruct you further.


Choose required services and fill in required details.

Small angle X-ray scattering
   
   
Single crystal X-ray diffraction
   
   
Complete dataset measurement:
   
   

BIOCEV - Structural mass spectrometry

If at any point of your measurements you need to use a different CMS core facility, you don´t need to fill out another request form. In such a case, please contact the facility you have requested access for and they will instruct you further.















BIOCEV sample(s) information

   


        
If Yes, please specify details in the Other specification field
            

        
If Yes, please specify expression host: